Optical microscopy for biology

Optical Microscopy

binocular microscopes with image-erecting prisms, and the first stereomicroscope (14). Early in the twentieth century, microscope manufacturers began parfocalizing objectives, allowing the image to remain in focus when the microscopist exchanged objectives on the rotating nosepiece. In 1824, Zeiss introduced a LeChatelier-style metallograph with infinitycorrected optics, but this method of corr...

CARS Microscopy For Biology and Medicine

Special Section: Microscopy in Biology

Fluorescence microscopy has become an indispensable tool in cell biology research due its exceptional specificity and ability to visualize subcellular structures with high contrast. It has highest impact when applied in 4D mode, i.e. when applied to record 3D image information as a function of time, since it allows the study of dynamic cellular processes in their native environment. The main is...

Optical-Resolution Photoacoustic Microscopy

Pure optical photoacoustic microscopy

The concept of pure optical photoacoustic microscopy(POPAM) was proposed based on optical rastering of a focused excitation beam and optically sensing the photoacoustic signal using a microring resonator fabricated by a nanoimprinting technique. After the refinements of the microring's working wavelength and in the resonator structure and mold fabrication, an ultrahigh Q factor of 3.0×10(5) was...